Dynamic Nuclear Polarization

We have an Oxford Hypersense dissolution DNP system connected to a Bruker 600 MHz spectrometer that houses a very high sensitivity 5mm 13C observe cryoprobe, thus providing maximum amplitude for these time limited signals.

Signals of 13C nuclei in the frozen sample are enhanced via transfer from electrons polarized by microwave irradiation. The polarized sample is then melted and dissolved, and transferred to a conventional NMR tube and typically 1D carbon data are acquired for the lifetime of the polarized state.  Generally this signal can be used to monitor enzyme reactions, transport into cells or other transient phenomena.